Transcription-dependent spreading of the Dal80 yeast GATA factor across the body of highly expressed genes.

ABSTRACT: GATA transcription factors are highly conserved among eukaryotes and play roles in transcription of genes implicated in cancer progression and hematopoiesis. However, although their consensus binding sites have been well defined in vitro, the in vivo selectivity for recognition by GATA factors remains poorly characterized. Using ChIP-Seq, we identified the Dal80 GATA factor targets in yeast. Our data reveal Dal80 binding to a large set of promoters, sometimes independently of GATA sites, correlating with nitrogen- and/or Dal80-sensitive gene expression. Strikingly, Dal80 was also detected across the body of promoter-bound genes, correlating with high expression. Mechanistic single-gene experiments showed that Dal80 spreading across gene bodies requires active transcription. Consistently, D

SUBMITTER: Ronsmans A 

PROVIDER: S-EPMC6413948 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature