Replication Study: The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44.

ABSTRACT: As part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Li et al., 2015), that described how we intended to replicate selected experiments from the paper 'The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44' (Liu et al., 2011). Here we report the results. We found the microRNA, miR-34a, was expressed at twice the level in CD44⁺ prostate cancer cells purified from xenograft tumors (LAPC4 cells) compared to CD44^- LAPC4 cells, whereas the original study reported miR-34a was underexpressed in CD44⁺ LAPC4 cells (Figure 1B; Liu et al., 2011). When LAPC4 cells engineered to express miR-34a were injected into mice, we did not observe changes in tumor growth or CD44 expression; however

SUBMITTER: Yan X 

PROVIDER: S-EPMC6414201 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature