Detection of splice isoforms and rare intermediates using multiplexed primer extension sequencing.

ABSTRACT: Targeted RNA sequencing (RNA-seq) aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-seq experiments. Here we present multiplexed primer extension sequencing (MPE-seq), an approach for targeted RNA-seq that uses complex pools of reverse-transcription primers to enable sequencing enrichment at user-selected locations across the genome. We targeted hundreds to thousands of pre-mRNA splice junctions and obtained high-precision detection of splice isoforms, including rare pre-mRNA splicing intermediates.

SUBMITTER: Xu H 

PROVIDER: S-EPMC6414223 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature