Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells.

ABSTRACT: A simple aspirin-inducible system has been developed and characterized in Escherichia coli by employing the P_sal promoter and SalR regulation system originally from Acinetobacter baylyi ADP1. Mutagenesis at the DNA binding domain (DBD) and chemical recognition domain (CRD) of the SalR protein in A. baylyi ADP1 suggests that the effector-free form, SalR_r, can compete with the effector-bound form, SalR_a, binding the P_sal promoter and repressing gene transcription. The induction of the P_sal promoter was compared in two different gene circuit designs: a simple regulation system (SRS) and positive autoregulation (PAR). Both regulatory circuits were induced in a dose-dependent manner in the presence

SUBMITTER: Chen JX 

PROVIDER: S-EPMC6414386 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature