Quantifying protein dynamics and stability in a living organism.

ABSTRACT: As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperature-jump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.

SUBMITTER: Feng R 

PROVIDER: S-EPMC6414637 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature