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ECM Decorated Electrospun Nanofiber for Improving Bone Tissue Regeneration.

ABSTRACT: Optimization of nanofiber surface properties can lead to enhanced tissue regeneration outcomes in the context of bone tissue engineering. Herein, we developed a facile strategy to decorate elctrospun nanofibers using extracellular matrix (ECM) in order to improve their performance for bone tissue engineering. Electrospun PLLA nanofibers (PLLA NF) were seeded with MC3T3-E1 cells and allowed to grow for two weeks in order to harvest a layer of ECM on nanofiber surface. After decellularization, we found that ECM was successfully preserved on nanofiber surface while maintaining the nanostructure of electrospun fibers. ECM decorated on PLLA NF is biologically active, as evidenced by its ability to enhance mouse bone marrow stromal cells (mBMSCs) adhesion, support cell proliferation and promote early stage osteogenic differentiation of mBMSCs. Compared to PLLA NF without ECM, mBMSCs grown on ECM/PLLA NF exhibited a healthier morphology, faster proliferation profile, and more robust osteogenic differentiation. Therefore, our study suggests that ECM decoration on electrospun nanofibers could serve as an efficient approach to improving their performance for bone tissue engineering.

SUBMITTER: Fu Y

PROVIDER: S-EPMC6414933 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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ECM Decorated Electrospun Nanofiber for Improving Bone Tissue Regeneration.

Fu Yong Y Liu Lili L Cheng Ruoyu R Cui Wenguo W

Polymers 20180306 3

Optimization of nanofiber surface properties can lead to enhanced tissue regeneration outcomes in the context of bone tissue engineering. Herein, we developed a facile strategy to decorate elctrospun nanofibers using extracellular matrix (ECM) in order to improve their performance for bone tissue engineering. Electrospun PLLA nanofibers (PLLA NF) were seeded with MC3T3-E1 cells and allowed to grow for two weeks in order to harvest a layer of ECM on nanofiber surface. After decellularization, we ...[more]

PMID: 30966307

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Project description:Crystalline platinum nanoparticles supported on carbon nanofibers were synthesized for use as an electrocatalyst for polymer electrolyte membrane fuel cells. The nanofibers were prepared by a method of electrospinning from polymer solution with subsequent pyrolysis. Pt nanoneedles supported on polyacrylonitrile pyrolyzed electrospun nanofibers were synthesized by chemical reduction of H2[PtCl6] in aqueous solution. The synthesized electrocatalysts were investigated using scanning, high resolution transmission and scanning transmission electron microscopies, EDX analysis and electron diffraction. The shape and the size of the electrocatalyst crystal Pt nanoparticles were controled and found to depend on the method of H2[PtCl6] reduction type and on conditions of subsequent thermal treatment. Soft Pt reduction by formic acid followed by 100 °C thermal treatment produced needle-shape Pt nanoparticles with a needle length up to 25 nm and diameter up to 5 nm. Thermal treatment of these nanoparticles at 500 °C resulted in partial sintering of the Pt needles. When formic acid was added after 24 h from the beginning of platinization, Pt reduction resulted in small-size spherical Pt nanoparticle of less than 10 nm in diameter. Reduction of H2[PtCl6], preadsorbed on electrospun nanofibers in formic acid with further treatment in H2 flow at 500 °C, resulted in intensive sintering of platinum particles, with formation of conglomerates of 50 nm in size, however, individual particles still retain a size of less than 10 nm. Electrochemically active surface area (ECSA) of Pt/C catalyst was measured by electrochemical hydrogen adsorption/desorption measurements in 0.5 M H2SO4. ECSA of needle-shape Pt nanoparticles was 25 m2 g-1. It increased up to 31 m2 g-1 after thermal treatment at 500 °C, likely, due to amorphous structures removal from carbon nanofibers and retaining of Pt nanoneedle morphology. ECSA of small-size spherical Pt nanoparticles was 26 m2 g-1. Further thermal treatment at 500 °C in vacuum decreased ECSA down to 20 m2 g-1 due to Pt sintering and Pt active sites deactivation. The thermal treatment of small-size spherical Pt nanoparticles in H2 flow at 500 °C produced agglomerates of Pt nanoparticles with ECSA of 14 m2 g-1.

Project description:IntroductionBone defect repair remains a major challenge in modern medicine. Although bone marrow mesenchymal stem cells (BMSCs) possess multilineage differentiation potential, traditional BMSC constructs are often limited in clinical applications due to insufficient osteogenic differentiation efficiency and inadequate vascularization.MethodsThis study developed an innovative bone tissue engineering strategy by combining BMSCs with gelatin/polycaprolactone (GT/PCL) nanofiber membranes to form cell sheets, which were then modified with endothelial cells (ECs) on the surface. The sheets were subsequently rolled into three-dimensional scaffolds to systematically evaluate their osteogenic potential and underlying mechanisms.ResuiltsResults showed that electrospun GT/PCL nanofiber membranes exhibited uniform fiber structure (diameter 200-500 nm), successfully mimicking the microstructure of natural extracellular matrix. In vitro experiments demonstrated that after 14 days of culture, EC modification significantly enhanced the osteogenic differentiation of BMSCs compared to unmodified controls, with approximately 3-fold increase in ALP expression (p < 0.05) and 2.5-fold increase in angiogenic factor VEGF expression (p < 0.01). Subcutaneous implantation in nude mice revealed superior bone formation capability of EC-modified constructs at both 4 and 8 weeks: micro-CT analysis showed bone density reaching 350 mg/cm3, bone surface area approaching 400 mm2, and bone volume fraction of approximately 20%, significantly higher than control groups (p < 0.0001). Immunohistochemical evaluation further confirmed more mature trabecular bone structure and richer vascular networks in EC-modified groups.DiscussionMechanistic studies revealed that EC modification promoted bone regeneration through three key pathways: optimization of local vascular microenvironment for improved nutrient supply, activation of intercellular synergistic signaling pathways, and reconstruction of physiological bone tissue microenvironment. This study not only validates the application value of this composite strategy in bone tissue engineering but also provides important theoretical basis for developing novel bone regeneration solutions.

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ECM Decorated Electrospun Nanofiber for Improving Bone Tissue Regeneration.

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ECM Decorated Electrospun Nanofiber for Improving Bone Tissue Regeneration.

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