Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing.

ABSTRACT:

Background

RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still limited.

Results

Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver, muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine (A-to-G) conversion. Most A-to-G sites were located in

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6415349 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature