Evaluation of Band-Selective HSQC and HMBC: Methodological Validation on the Cyclosporin Cyclic Peptide and Application for Poly(3-hydroxyalkanoate)s Stereoregularity Determination.

ABSTRACT: Band-selective (bs) HSQC, improving spectral resolution by restriction of the heteronuclear dimension without inducing spectral folding, has been recently used for polymer tacticity determination. Herein is reported an evaluation of various bs-HSQC and bs-HMBC sequences, first from a methodological point of view (selectivity, dependence to INEPT interpulse delay or relaxation delay), using the cyclic peptide cyclosporin selected as a model compound, and then from an applicative approach, comparing tacticity determined from bs-HSQC and bs-HMBC experiments to the one obtained from 1D ¹³C{¹H} on poly(3-hydroxyalkanoate)s samples. For HSQC sequences, the ¹³C selectivity scheme consisting in substituting a ¹³C broadband refocalization by a selective one revealed

SUBMITTER: Caytan E 

PROVIDER: S-EPMC6415406 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature