Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma.

ABSTRACT:

Background

The MYBL2 gene, a highly conserved member of the Myb transcription-factor family, has been implicated in the genesis and progression of many types of tumors.

Methods

We analyzed the expression of MYBL2 and Ki67 in tissue samples of esophageal squamous-cell carcinoma (ESCC) patients by immunohistochemistry. We further analyzed the effect of MYBL2 on cell proliferation and DNA replication using a CCK8 assay, 5-ethynyl-2'-deoxyuridine-retention assay, flow-cytometry analysis, real-time quantitative PCR, Western blot, and a xenograft model of ESCC cells in nude mice.

Results

MYBL2 expression was significantly higher in ESCC tissue when compared to the adjacent normal tissue (P=0.007). MYBL2 was found to be positively correlated with Ki67 (γ=0.286,

SUBMITTER: Qin H 

PROVIDER: S-EPMC6415733 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature