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Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches.

ABSTRACT: MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Detection of microRNAs can be arduous and expensive and often requires amplification, labeling, or radioactive probes. Here, we report a single-step, nonenzymatic microRNA detection assay using conformationally responsive DNA nanoswitches. Termed miRacles (microRNA-activated conditional looping of engineered switches), our assay has subattomole sensitivity and single-nucleotide specificity using an agarose gel electrophoresis readout. We detect cellular microRNAs from nanogram-scale RNA extracts of differentiating muscle cells and multiplex our detection for several microRNAs from one biological sample. We demonstrate 1-hour detection without expensive equipment or reagents, making this assay a compelling alternative to quantitative polymerase chain reaction and Northern blotting.

SUBMITTER: Chandrasekaran AR

PROVIDER: S-EPMC6415953 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches.

Chandrasekaran Arun Richard AR MacIsaac Molly M Dey Paromita P Levchenko Oksana O Zhou Lifeng L Andres Madeline M Dey Bijan K BK Halvorsen Ken K

Science advances 20190313 3

MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Detection of microRNAs can be arduous and expensive and often requires amplification, labeling, or radioactive probes. Here, we report a single-step, nonenzymatic microRNA detection assay using conformationally responsive DNA nanoswitches. Termed miRacles (microRNA-activated conditional looping of engineered switches), our assay has subattomole sensitivity and single-nucleotide specificity using an ag ...[more]

PMID: 30891499

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Dataset Information

Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches.

Publications

Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches.

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