CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)^G93A ALS mice originate from astrocytes and neurons and carry misfolded SOD1.

ABSTRACT: Extracellular vesicles (EVs) are secreted by myriad cells in culture and also by unicellular organisms, and their identification in mammalian fluids suggests that EV release also occurs at the organism level. However, although it is clearly important to better understand EVs' roles in organismal biology, EVs in solid tissues have received little attention. Here, we modified a protocol for EV isolation from primary neural cell culture to collect EVs from frozen whole murine and human neural tissues by serial centrifugation and purification on a sucrose gradient. Quantitative proteomics comparing brain-derived EVs from nontransgenic (NTg) and a transgenic amyotrophic lateral sclerosis (ALS) mouse model, superoxide dismutase 1 (SOD1)^G93A, revealed that these EVs contain canonical e

SUBMITTER: Silverman JM 

PROVIDER: S-EPMC6416428 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature