DSab-origin: a novel IGHD sensitive VDJ mapping method and its application on antibody response after influenza vaccination.

ABSTRACT:

Background

Functional antibody genes are often assembled by VDJ recombination and then diversified by somatic hypermutation. Identifying the combination of sourcing germline genes is critical to understand the process of antibody maturation, which may facilitate the diagnostics and rapid generation of human monoclonal antibodies in therapeutics. Despite of successful efforts in V and J fragment assignment, method in D segment tracing remains weak for immunoglobulin heavy diversity (IGHD).

Results

In this paper, we presented a D-sensitive mapping method called DSab-origin with accuracies around 90% in human monoclonal antibody data and average 95.8% in mouse data. Besides, DSab-origin achieved the best performance in holistic prediction of VDJ segments assignment comparing wi

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC6417009 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature