Collagen density regulates the activity of tumor-infiltrating T cells.

ABSTRACT:

Background

Tumor progression is accompanied by dramatic remodeling of the surrounding extracellular matrix leading to the formation of a tumor-specific ECM, which is often more collagen-rich and of increased stiffness. The altered ECM of the tumor supports cancer growth and metastasis, but it is unknown if this effect involves modulation of T cell activity. To investigate if a high-density tumor-specific ECM could influence the ability of T cells to kill cancer cells, we here studied how T cells respond to 3D culture in different collagen densities.

Methods

T cells cultured in 3D conditions surrounded by a high or low collagen density were imaged using confocal fluorescent microscopy. The effects of the different collagen densities on T cell proliferation, survival, and diff

SUBMITTER: Kuczek DE 

PROVIDER: S-EPMC6417085 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature