Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans.

ABSTRACT: Blood or biopsies are often used to characterize metabolites that are modulated by exercising muscle. However, blood has inputs derived from multiple tissues, biopsies cannot discriminate between secreted and intracellular metabolites, and their invasive nature is challenging for frequent collections in sensitive populations (e.g., children and pregnant women). Thus, minimally invasive approaches to interstitial fluid (IF) metabolomics would be valuable. A catheter was designed to collect IF from the gastrocnemius muscle of acutely anesthetized adult male rats at rest or immediately following 20 min of exercise (~60% of maximal O₂ uptake). Nontargeted, gas chromatography-time-of-flight mass spectrometry analysis was used to detect 299 metabolites, including nonannotated metaboli

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6417688 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature