PPARα-Mediated Positive-Feedback Loop Contributes to Cold Exposure Memory.

ABSTRACT: Fluctuations in food availability and shifts in temperature are typical environmental changes experienced by animals. These environmental shifts sometimes portend more severe changes; e.g., chilly north winds precede the onset of winter. Such telltale signs may be indicators for animals to prepare for such a shift. Here we show that HEK293A cells, cultured under starvation conditions, can "memorize" a short exposure to cold temperature (15 °C), which was evidenced by their higher survival rate compared to cells continuously grown at 37 °C. We refer to this phenomenon as "cold adaptation". The cold-exposed cells retained high ATP levels, and addition of etomoxir, a fatty acid oxidation inhibitor, abrogated the enhanced cell survival. In our standard protocol, cold adaptation required linole

SUBMITTER: Alfaqaan S 

PROVIDER: S-EPMC6418111 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature