Ultra-fast and automated immunohistofluorescent multistaining using a microfluidic tissue processor.

ABSTRACT: Multistaining of a tissue section targeting multiple markers allows to reveal complex interplays in a tumor environment. However, the resource-intensive and impractically long nature of iterative multiplexed immunostainings prohibits its practical implementation in daily routine, even when using work-flow automation systems. Here, we report a fully automated and ultra-fast multistaining using a microfluidic tissue processor (MTP) in as short as 20 minutes per marker, by immunofluorescent staining employing commercially available tyramide signal amplification polymer precipitation by horse-radish peroxidase (HRP) activation. The reported duration includes (i) 15 minutes for the entire fluidic exchange and reagent incubation necessary for the immunostaining and (ii) 5 minutes for the heat-in

SUBMITTER: Cappi G 

PROVIDER: S-EPMC6418167 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature