Forkhead box O3 promotes colon cancer proliferation and drug resistance by activating MDR1 expression.

ABSTRACT:

Background

Globally, colon cancer (CC) is the third reason of tumor-related deaths. Previous reports indicate that Forkhead box O3 (FOXO3) is involved in the development of various tumors and may have different effects depending upon the types of tumors. Hence, this study was to examine the effects of FOXO3 on CC cells and uncover the possible mechanisms.

Methods

MTT and cell count assay were applied to analyze the viability of transfected CC cells. rVista, dual luciferase reporter assay, and chromatin immunoprecipitation assay were used to identify the downstream target of FOXO3 in HCT116 cells. The mRNA and protein abundance of FOXO3 and MDR1 were determined by quantitative PCR and Western blot, respectively.

Results

Forkhead box O3 stimulated the proliferation of b

SUBMITTER: Gao Z 

PROVIDER: S-EPMC6418361 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature