Targeted in vivo epigenome editing of H3K27me3.

ABSTRACT:

Background

Epigenetic modifications have a central role in transcriptional regulation. While several studies using next-generation sequencing have revealed genome-wide associations between epigenetic modifications and transcriptional states, a direct causal relationship at specific genomic loci has not been fully demonstrated, due to a lack of technology for targeted manipulation of epigenetic modifications. Recently, epigenome editing techniques based on the CRISPR-Cas9 system have been reported to directly manipulate specific modifications at precise genomic regions. However, the number of editable modifications as well as studies applying these techniques in vivo is still limited.

Results

Here, we report direct modification of the epigenome in medaka (Japanese killifish,

SUBMITTER: Fukushima HS 

PROVIDER: S-EPMC6419334 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature