Rational protein design of Bacillus sp. MN chitosanase for altered substrate binding and production of specific chitosan oligomers.

ABSTRACT:

Background

Partially acetylated chito-oligosaccharides (paCOS) have a variety of potential applications in different fields, but to harness their benefits, pure paCOS or well-defined paCOS mixtures are essential. For example, if one could produce fully acetylated (A₄) and fully deacetylated (D₄) tetramers in abundance, all possible variants of tetrameric paCOS could be generated reliably from them. A promising approach for generating defined paCOS is by enzymatic depolymerization of chitosan polymers using chitosanases, since these enzymes' subsite specificities directly influence the composition of the paCOS produced; however, enzymatic production of e.g. D₄ is challenging because the substrate is generally hydrolyzed further by most chitosanases.

SUBMITTER: Gercke D 

PROVIDER: S-EPMC6419424 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature