CoCl₂ simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis.

ABSTRACT:

Background/aims

Hypoxia microenvironment plays a crucial role during tumor progression and it tends to exhibit poor prognosis and make resistant to various conventional therapies. HIF-1α acts as an important transcriptional regulator directly or indirectly associated with genes involved in cell proliferation, angiogenesis, apoptosis and energy metabolism during tumor progression in hypoxic microenvironment. This study was aimed to investigate the expression pattern of the hypoxia associated genes and their association during breast cancer progression under hypoxic microenvironment in breast cancer cells.

Methods

Cell proliferation in MCF-7 and MDA-MB-231 cell lines treated with different concentration of CoCl₂ was analyzed by MTT assay. Flow cytometry was performe

SUBMITTER: Rana NK 

PROVIDER: S-EPMC6419504 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature