Efficient recovery and enrichment of infectious rotavirus using separation with antibody-integrated graphite-encapsulated magnetic nanobeads produced by argon/ammonia gas plasma technology.

ABSTRACT:

Background

Rotavirus is the representative cause of severe acute gastroenteritis in young children. A characteristic feature of rotavirus is low infectious dose and robustness of the virion, suggesting sanitation and hygiene will have little impact. Thus, development of a vaccine should be given priority. Efficient capture of infectious viruses is an important step in generating a vaccine. Previously, antibody-integrated magnetic nanobeads (MNBs) have been developed for virus capture. This study examines the applicability of this method for infectious rotavirus recovery and enrichment.

Materials and methods

Graphite-encapsulated MNBs were treated with radio frequency (RF) excited Ar/NH₃ gas mixture plasma to introduce amino groups onto their surfaces. Rotavirus vi

SUBMITTER: Yamashiro R 

PROVIDER: S-EPMC6419588 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature