Determination of two fluoroquinolones and their combinations with hyaluronan effect in in vitro canine cartilage explants.

ABSTRACT:

Background

Previous studies reported the effect of enrofloxacin (Enro) and marbofloxacin (Mar) on cell death and alteration of the key genes involved in catabolic and anabolic processes and demonstrated the beneficial effects of hyaluronan (HA) combined with fluoroquinolones (FQs) on primary canine chondrocytes. This study further determines the effects of these treatments on canine cartilage explants in both normal and interleukin-1 beta (IL-1β)-stimulated conditions.

Methods

We examined sulfate glycosaminoglycan (s-GAG) release, uronic acid (UA) content, and safranin-O staining, as well as the expression patterns of inflammatory, extracellular matrix (ECM) component and enzymes.

Results

Enro treatment alone effectively stimulated proteoglycan anabolism by increasing

SUBMITTER: Siengdee P 

PROVIDER: S-EPMC6419719 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature