Early detection of colorectal cancer based on presence of methylated syndecan-2 (SDC2) in stool DNA.

ABSTRACT:

Background

Colorectal cancer (CRC) screening can effectively reduce disease-related mortality by detecting CRC at earlier stages. We have previously demonstrated that the presence of SDC2 methylation in stool DNA is significantly associated with the occurrence of CRC regardless of clinical stage. The aim of this study was to evaluate the clinical performance of stool DNA-based SDC2 methylation test for CRC.

Methods

Aberrant SDC2 methylation in stool-derived DNA was measured by linear target enrichment (LTE)-quantitative methylation-specific real-time PCR (qMSP). Duplicate reactions of meSDC2 LTE-qMSP test were performed for stool samples obtained from CRC patients representing all stages (0-IV) and asymptomatic individuals who were subsequently underwent colonoscopy examinat

SUBMITTER: Han YD 

PROVIDER: S-EPMC6419806 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature