Immobilization of an Endo-β-N-acetylglucosaminidase for the Release of Bioactive N-glycans.

ABSTRACT: As more is learned about glycoproteins' roles in human health and disease, the biological functionalities of N-linked glycans are becoming more relevant. Protein deglycosylation allows for the selective release of N-glycans and facilitates glycoproteomic investigation into their roles as prebiotics or anti-pathogenic factors. To increase throughput and enzyme reusability, this work evaluated several immobilization methods for an endo-β-N-acetylglucosaminidase recently discovered from the commensal Bifidobacterium infantis. Ribonuclease B was used as a model glycoprotein to compare N-glycans released by the free and immobilized enzyme. Amino-based covalent method showed the highest enzyme immobilization. Relative abundance of N-glycans and enzyme activity were determined using matrix-assist

SUBMITTER: Cohen JL 

PROVIDER: S-EPMC6419974 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature