Novel multiplex assay for profiling influenza antibodies in breast milk and serum of mother-infant pairs.

ABSTRACT: Background: During early life, systemic protection to influenza is passively provided by transplacental transfer of IgG antibodies and oral and gastrointestinal mucosal protection via breast milk (BM) containing predominantly IgA. Immune imprinting, influenced by initial exposure of the infant immune system to influenza, has recently been recognized as an important determinant of future influenza immune responses. Methods: We utilized stored frozen BM from a prospective birth cohort to assess immune factors in human milk. The earliest available BM and a paired, timed serum sample was assessed from each of  7 mothers. Paired infant serum samples were assayed at up to three time points during the first 12 months of life, one prior to assumed disappearance of transplacentally tr

SUBMITTER: Jarvinen KM 

PROVIDER: S-EPMC6419979 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature