NG-Tax, a highly accurate and validated pipeline for analysis of 16S rRNA amplicons from complex biomes.

ABSTRACT: Background: Massive high-throughput sequencing of short, hypervariable segments of the 16S ribosomal RNA (rRNA) gene has transformed the methodological landscape describing microbial diversity within and across complex biomes. However, several studies have shown that the methodology rather than the biological variation is responsible for the observed sample composition and distribution. This compromises meta-analyses, although this fact is often disregarded. Results: To facilitate true meta-analysis of microbiome studies, we developed NG-Tax, a pipeline for 16S rRNA gene amplicon sequence analysis that was validated with different mock communities and benchmarked against QIIME as a frequently used pipeline. The microbial composition of 49 independently amplified mock s

SUBMITTER: Ramiro-Garcia J 

PROVIDER: S-EPMC6419982 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature