H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids.

ABSTRACT: Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining¹. HR supresses tumorigenesis¹, but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present². Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding protein 1(53BP1) (refs. ^2-5), but it remains unknown how BRCA1 function is limited to the S and G2 phases. We show that BRCA1 recruitment requires recognition of histone H4 unmethylated at lysine 20 (H4K20me0), linking DSB repair pathway choice directly to sister chromatid availability. We identify the ankyrin repeat domain of BRCA1-associated RING domain protein 1

SUBMITTER: Nakamura K 

PROVIDER: S-EPMC6420097 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature