Host Gene Expression in Nose and Blood for the Diagnosis of Viral Respiratory Infection.

ABSTRACT:

Background

Recently there has been a growing interest in the potential for host transcriptomic analysis to augment the diagnosis of infectious diseases.

Methods

We compared nasal and blood samples for evaluation of the host transcriptomic response in children with acute respiratory syncytial virus (RSV) infection, symptomatic non-RSV respiratory virus infection, asymptomatic rhinovirus infection, and virus-negative asymptomatic controls. We used nested leave-one-pair-out cross-validation and supervised principal components analysis to define small sets of genes whose expression patterns accurately classified subjects. We validated gene classification scores using an external data set.

Results

Despite lower quality of nasal RNA, the number of genes detected by microarr

SUBMITTER: Yu J 

PROVIDER: S-EPMC6420164 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature