Ontology highlight
ABSTRACT:
SUBMITTER: Hwang B
PROVIDER: S-EPMC6420643 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Nature communications 20190315 1
Determining cell lineage and function is critical to understanding human physiology and pathology. Although advances in lineage tracing methods provide new insight into cell fate, defining cellular diversity at the mammalian level remains a challenge. Here, we develop a genome editing strategy using a cytidine deaminase fused with nickase Cas9 (nCas9) to specifically target endogenous interspersed repeat regions in mammalian cells. The resulting mutation patterns serve as a genetic barcode, whic ...[more]