Single-cell RNA-seq reveals dynamic transcriptome profiling in human early neural differentiation.

ABSTRACT:

Background

Investigating cell fate decision and subpopulation specification in the context of the neural lineage is fundamental to understanding neurogenesis and neurodegenerative diseases. The differentiation process of neural-tube-like rosettes in vitro is representative of neural tube structures, which are composed of radially organized, columnar epithelial cells and give rise to functional neural cells. However, the underlying regulatory network of cell fate commitment during early neural differentiation remains elusive.

Results

In this study, we investigated the genome-wide transcriptome profile of single cells from six consecutive reprogramming and neural differentiation time points and identified cellular subpopulations present at each differentiation stage. Based on

SUBMITTER: Shang Z 

PROVIDER: S-EPMC6420650 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature