Consideration of sample motion in cryo-tomography based on alignment residual interpolation.

ABSTRACT: Recently, it has been shown that the resolution in cryo-tomography could be improved by considering the sample motion in tilt-series alignment and reconstruction, where a set of quadratic polynomials were used to model this motion. One requirement of this polynomial method is the optimization of a large number of parameters, which may limit its practical applicability. In this work, we propose an alternative method for modeling the sample motion. Starting from the standard fiducial-based tilt-series alignment, the method uses the alignment residual as local estimates of the sample motion at the 3D fiducial positions. Then, a scattered data interpolation technique characterized by its smoothness and a closed-form solution is applied to model the sample motion. The motion model is then integ

SUBMITTER: Fernandez JJ 

PROVIDER: S-EPMC6420838 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature