Molecular Classification of Lymph Node Metastases Subtypes Predict for Survival in Head and Neck Cancer.

ABSTRACT:

Purpose

In advanced stage head and neck squamous cell cancers (HNSCC), approximately half of the patients with lymph node metastases (LNM) are not cured. Given the heterogeneous outcomes in these patients, we profiled the expression patterns of LNMs to identify the biological factors associated with patient outcomes.Experimental Design: We performed mRNAseq and miRNAseq on 72 LNMs and 29 matched primary tumors from 34 patients with HNSCC. Clustering identified molecular subtypes in LNMs and in primary tumors. Prediction Analysis of Microarrays algorithm identified a 73-gene classifier that distinguished LNM subtypes. Gene-set enrichment analysis identified pathways upregulated in LNM subtypes.

Results

Integrative clustering identified three distinct LNM subtypes: (i)

SUBMITTER: Huang L 

PROVIDER: S-EPMC6420850 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature