Transcription and Remodeling Produce Asymmetrically Unwrapped Nucleosomal Intermediates.

ABSTRACT: Nucleosomes are disrupted during transcription and other active processes, but the structural intermediates during nucleosome disruption in vivo are unknown. To identify intermediates, we mapped subnucleosomal protections in Drosophila cells using Micrococcal Nuclease followed by sequencing. At the first nucleosome position downstream of the transcription start site, we identified unwrapped intermediates, including hexasomes that lack either proximal or distal contacts. Inhibiting topoisomerases or depleting histone chaperones increased unwrapping, whereas inhibiting release of paused RNAPII or reducing RNAPII elongation decreased unwrapping. Our results indicate that positive torsion generated by elongating RNAPII causes transient loss of histone-DNA contacts. Using this mapping approach,

SUBMITTER: Ramachandran S 

PROVIDER: S-EPMC6421108 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature