Transcriptional Analysis of the Human IgE-Expressing Plasma Cell Differentiation Pathway.

ABSTRACT: IgE is secreted by plasma cells (PCs) and is central to allergic disease. Using an ex vivo tonsil B cell culture system, which mimics the Th2 responses in vivo, we have recently characterized the development pathway of human IgE-expressing PCs. In this system, as in mice, we reported the predisposition of IgE-expressing B cells to differentiate into PCs. To gain a comprehensive understanding of the molecular events involved in the differentiation of human IgE⁺ B cells into PCs we have used the Illumina HumanHT-12 v4 Expression BeadChip array to analyse the gene expression profile of ex vivo generated human IgE⁺ B cells at various stages of their differentiation into PCs. We also compared the transcription profiles of IgE⁺ and IgG1⁺

SUBMITTER: Ramadani F 

PROVIDER: S-EPMC6421273 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature