RNA-sequencing in ophthalmology research: considerations for experimental design and analysis.

ABSTRACT: High-throughput, massively parallel sequence analysis has revolutionized the way that researchers design and execute scientific investigations. Vast amounts of sequence data can be generated in short periods of time. Regarding ophthalmology and vision research, extensive interrogation of patient samples for underlying causative DNA mutations has resulted in the discovery of many new genes relevant to eye disease. However, such analysis remains functionally limited. RNA-sequencing accurately snapshots thousands of genes, capturing many subtypes of RNA molecules, and has become the gold standard for transcriptome gene expression quantification. RNA-sequencing has the potential to advance our understanding of eye development and disease; it can reveal new candidates to improve our molecular d

SUBMITTER: Owen N 

PROVIDER: S-EPMC6421592 | biostudies-literature | 2019 Jan-Dec

REPOSITORIES: biostudies-literature