ECT2/PSMD14/PTTG1 axis promotes the proliferation of glioma through stabilizing E2F1.

ABSTRACT:

Background

Epithelial cell transformation sequence 2 (ECT2) is upregulated in glioma and promotes glioma cell proliferation. A preliminary experiment showed a positive correlation between ECT2 and pituitary tumor-transforming gene 1 (PTTG1). The aim of this study was to explore how ECT2 affects PTTG1 to influence the proliferation of glioma cells.

Methods

The expression of ECT2 in glioma was detected by western blot and reverse transcription PCR. The effect of ECT2 on glioma proliferation was examined using cell proliferation-related assays and in vivo experiments. The effect of ECT2 on the stability of E2F transcription factor 1 (E2F1) and the expression of PTTG1 were examined by western blot, co-immunoprecipitation, and in vivo ubiquitination assays.

Results

ECT2 wa

SUBMITTER: Zhi T 

PROVIDER: S-EPMC6422441 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature