Polymicrobial synergy within oral biofilm promotes invasion of dendritic cells and survival of consortia members.

ABSTRACT: Years of human microbiome research have confirmed that microbes rarely live or function alone, favoring diverse communities. Yet most experimental host-pathogen studies employ single species models of infection. Here, the influence of three-species oral microbial consortium on growth, virulence, invasion and persistence in dendritic cells (DCs) was examined experimentally in human monocyte-derived dendritic cells (DCs) and in patients with periodontitis (PD). Cooperative biofilm formation by Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis was documented in vitro using growth models and scanning electron microscopy. Analysis of growth rates by species-specific 16s rRNA probes revealed distinct, early advantages to consortium growth for S. gordonii and F. nucleat

SUBMITTER: El-Awady A 

PROVIDER: S-EPMC6423025 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature