Localization of Secretory Mucins MUC5AC and MUC5B in Normal/Healthy Human Airways.

ABSTRACT:

Rationale

MUC5AC and MUC5B are the predominant gel-forming mucins in the mucus layer of human airways. Each mucin has distinct functions and site-specific expression. However, the regional distribution of expression and cell types that secrete each mucin in normal/healthy human airways are not fully understood.

Objectives

To characterize the regional distribution of MUC5B and MUC5AC in normal/healthy human airways and assess which cell types produce these mucins, referenced to the club cell secretory protein (CCSP).

Methods

Multiple airway regions from 16 nonsmoker lungs without a history of lung disease were studied. MUC5AC, MUC5B, and CCSP expression/colocalization were assessed by RNA in situ hybridization and immunohistochemistry in five lungs with histologically

SUBMITTER: Okuda K 

PROVIDER: S-EPMC6423099 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature