Near real-time enumeration of live and dead bacteria using a fibre-based spectroscopic device.

ABSTRACT: A rapid, cost-effective and easy method that allows on-site determination of the concentration of live and dead bacterial cells using a fibre-based spectroscopic device (the optrode system) is proposed and demonstrated. Identification of live and dead bacteria was achieved by using the commercially available dyes SYTO 9 and propidium iodide, and fluorescence spectra were measured by the optrode. Three spectral processing methods were evaluated for their effectiveness in predicting the original bacterial concentration in the samples: principal components regression (PCR), partial least squares regression (PLSR) and support vector regression (SVR). Without any sample pre-concentration, PCR achieved the most reliable results. It was able to quantify live bacteria from 10⁸ down to 1

SUBMITTER: Ou F 

PROVIDER: S-EPMC6423134 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature