Responsiveness of hypothalamo-pituitary-adrenal axis to leptin is impaired in diet-induced obese rats.

ABSTRACT:

Background/objectives

Diet-induced obese (DIO) rats have altered stress (HPA) axis activity compared to diet-resistant (DR) rats when chronically exposed to a high-fat (HF) diet. Since stress axis is tightly regulated by leptin, an adipocyte-secreted hormone that is important for controlling body weight, we hypothesized that leptin action is impaired in DIO rats leading to alterations in HPA axis activity.

Subjects/methods

We intraperitoneally injected selectively bred DIO and DR rats with either saline or recombinant rat leptin. HPA axis activity was assessed by measuring norepinephrine (NE) in the paraventricular nucleus (PVN), corticotropin-releasing hormone (CRH) in the median eminence, and serum corticosterone (CORT). To test if HF exposure duration and the correspondin

SUBMITTER: Shin AC 

PROVIDER: S-EPMC6423225 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature