Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming.

ABSTRACT: Swarming in Pseudomonas aeruginosa is a coordinated movement of bacteria over semisolid surfaces (0.5%-0.7% agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching. However, the swarm patterns typically vary depending upon the experimental design. In the present study, we show that the pattern characteristics of P. aeruginosa swarm are highly environment dependent. We define several quantifiable, macroscale features of the swarm to study the plasticity of the swarm, observed across different nutrient formulations. Furthermore, through a targeted screen of 113 two-component system (TCS) loci of the P. aeruginosa strain PA14, we show that forty-four TCS genes regulate swarming in PA14 in a contextual fashion. However, only four TCS genes-fleR,

SUBMITTER: Kollaran AM 

PROVIDER: S-EPMC6423354 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature