Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers.

ABSTRACT: Twenty inter-simple sequence repeat (ISSR) and twenty two start codon targeted (SCoT) primers were employed to analyze genetic diversity and population structure among 52 Trichosanthes dioica Roxb. accessions collected from nine different eco-geographical regions of India. ISSR markers proved to be more informative in genetic diversity assessment and produced higher mean number of polymorphic bands (15.25 with 95.96% polymorphism) and polymorphic information content (PIC) value (0.47) compared to SCoT markers (12.55 polymorphic bands with 92.20% polymorphism and PIC: 0.45). Total genetic diversity (Ht) and genetic diversity within populations (Hs) in T. dioica accessions was found to be very high (0.45 and 0.43, respectively). AMOVA analysis also revealed higher

SUBMITTER: Kumar J 

PROVIDER: S-EPMC6423492 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature