Detection of Plasmodium falciparum infected Anopheles gambiae using near-infrared spectroscopy.

ABSTRACT:

Background

Large-scale surveillance of mosquito populations is crucial to assess the intensity of vector-borne disease transmission and the impact of control interventions. However, there is a lack of accurate, cost-effective and high-throughput tools for mass-screening of vectors.

Methods

A total of 750 Anopheles gambiae (Keele strain) mosquitoes were fed Plasmodium falciparum NF54 gametocytes through standard membrane feeding assay (SMFA) and afterwards maintained in insectary conditions to allow for oocyst (8 days) and sporozoite development (14 days). Thereupon, each mosquito was scanned using near infra-red spectroscopy (NIRS) and processed by quantitative polymerase chain reaction (qPCR) to determine the presence of infection and infection load. The spectra collected w

SUBMITTER: Maia MF 

PROVIDER: S-EPMC6423776 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature