Elucidating the mechanism of action of domatinostat (4SC-202) in cutaneous T cell lymphoma cells.

ABSTRACT:

Background

Targeting epigenetic modifiers is effective in cutaneous T cell lymphoma (CTCL). However, there is a need for further improvement of this therapeutic approach. Here, we compared the mode of action of romidepsin (FK228), an established class I histone deacetylase inhibitor, and domatinostat (4SC-202), a novel inhibitor of class I HDACs, which has been reported to also target the lysine-specific histone demethylase 1A (LSD1).

Methods

We performed MTS assays and flow cytometric analyses of propidium iodide or annexin V-stained cells to assess drug impact on cellular proliferation, cell cycle distribution, and survival. Histone acetylation and methylation as well as caspase activation was analyzed by immunoblot. Gene expression analysis was performed using NanosString

SUBMITTER: Wobser M 

PROVIDER: S-EPMC6423872 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature