Rapid and Simple Detection of Viable Foodborne Pathogen Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus (S. aureus) contamination in food safety has become a worldwide health problem. In this work, we utilized RNA one-step detection of denaturation bubble-mediated Strand Exchange Amplification (SEA) method to realize the detection of viable foodborne pathogen S. aureus. A pair of S. aureus specific primers were designed for the SEA reaction by targeting hypervariable V2 region of 16S rDNA and the amplification reaction was finished about 1 h. The results of amplification reaction could be observed by the naked eyes with a significant color change from light yellow to red to realize the colorimetric detection of S. aureus. Therefore, there only required an isothermal water bath, which was very popular for areas with limited resources. I

SUBMITTER: Liu C 

PROVIDER: S-EPMC6424009 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature