Evaluation of ¹¹¹In-DOTA-5D3, a Surrogate SPECT Imaging Agent for Radioimmunotherapy of Prostate-Specific Membrane Antigen.

ABSTRACT: 5D3 is a new high-affinity murine monoclonal antibody specific for prostate-specific membrane antigen (PSMA). PSMA is a target for the imaging and therapy of prostate cancer. ¹¹¹In-labeled antibodies have been used as surrogates for ¹⁷⁷Lu/⁹⁰Y-labeled therapeutics. We characterized ¹¹¹In-DOTA-5D3 by SPECT/CT imaging, tissue biodistribution studies, and dosimetry. Methods: Radiolabeling, stability, cell uptake, and internalization of ¹¹¹In-DOTA-5D3 were performed by established techniques. Biodistribution and SPECT imaging were done on male nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice bearing human PSMA(+) PC3 PIP and PSMA(-) PC3 flu prostate cancer xenografts on the upper right and left flanks, respectively,

SUBMITTER: Banerjee SR 

PROVIDER: S-EPMC6424233 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature