Exploring the bacteriome in anthropophilic ticks: To investigate the vectors for diagnosis.

ABSTRACT:

Objective

The aim of this study was to characterize the bacterial microbiome of hard ticks with affinity to bite humans in La Rioja (North of Spain).

Methods

A total of 88 adult ticks (22 Rhipicephalus sanguineus sensu lato, 27 Haemaphysalis punctata, 30 Dermacentor marginatus and 9 Ixodes ricinus) and 120 I. ricinus nymphs (CRETAV collection, La Rioja, Spain), representing the main anthropophilic species in our environment, were subjected to a metagenomic analysis of the V3-V4 region of the 16S rRNA gene using an Illumina MiSeq platform. Data obtained with Greengenes database were refined with BLAST. Four groups of samples were defined, according to the four tick species.

Results

Proteobacteria was the predominant phylum observed in all groups. Gammaproteobacteria wa

SUBMITTER: Portillo A 

PROVIDER: S-EPMC6424421 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature