Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential.

ABSTRACT: Undifferentiated human embryonic stem cells have a distinct morphology (hESC). Changes in cell morphology during culture can be indicative of differentiation. hESC, maintained in diverse medias, demonstrated alterations in morphological parameters and subsequent alterations in underlying transcript expression and lineage differentiation. Analysis of morphological parameters showed distinct and significant differences between the undefined, less defined and Xeno-free medias while still maintaining pluripotency markers. This suggested that the less defined media may be creating dynamic instability in the cytoskeleton, with the cytoskeleton becoming more stabilised in the Xeno-free media as demonstrated by smaller and rounder cells. Examination of early lineage markers during undirected diffe

SUBMITTER: Harkness L 

PROVIDER: S-EPMC6424453 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature