Network Properties Revealed during Multi-Scale Calcium Imaging of Seizure Activity in Zebrafish.

ABSTRACT: Seizures are characterized by hypersynchronization of neuronal networks. Understanding these networks could provide a critical window for therapeutic control of recurrent seizure activity, i.e., epilepsy. However, imaging seizure networks has largely been limited to microcircuits in vitro or small "windows" in vivo. Here, we combine fast confocal imaging of genetically encoded calcium indicator (GCaMP)-expressing larval zebrafish with local field potential (LFP) recordings to study epileptiform events at whole-brain and single-neuron levels in vivo. Using an acute seizure model (pentylenetetrazole, PTZ), we reliably observed recurrent electrographic ictal-like events associated with generalized activation of all major brain regions and uncovered a well-preserved anteri

SUBMITTER: Liu J 

PROVIDER: S-EPMC6424556 | biostudies-literature | 2019 Jan-Feb

REPOSITORIES: biostudies-literature