ARTC2.2/P2RX7 Signaling during Cell Isolation Distorts Function and Quantification of Tissue-Resident CD8⁺ T Cell and Invariant NKT Subsets.

ABSTRACT: Peripheral invariant NKT cells (iNKT) and CD8⁺ tissue-resident memory T cells (T_RM) express high levels of the extracellular ATP receptor P2RX7 in mice. High extracellular ATP concentrations or NAD-mediated P2RX7 ribosylation by the enzyme ARTC2.2 can induce P2RX7 pore formation and cell death. Because both ATP and NAD are released during tissue preparation for analysis, cell death through these pathways may compromise the analysis of iNKT and CD8⁺ T_RM Indeed, ARTC2.2 blockade enhanced recovery of viable liver iNKT and T_RM The expression of ARTC2.2 and P2RX7 on distinct iNKT subsets and T_RM is unclear, however, as is the impact of recovery from other nonlymphoid sites. In this study, we performed a comprehensive analysis of

SUBMITTER: Borges da Silva H 

PROVIDER: S-EPMC6424602 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature